Simultaneous Spectrophotometric Estimation of Tenofovir Disoproxil Fumarate and Lamivudine in Three Component Tablet Formulation Containing Efavirenz
نویسندگان
چکیده
Three UV spectrophotometric methods have been developed, simultaneous equation method, multicomponent analysis (II) and derivative spectroscopy method (III). The absorption maxima of the drugs were found to be 247, 259 and 272 nm, respectively for efavirenz, tenofovir disoproxil fumarate and lamivudine in methanol:water (50:50) solvent system. Efavirenz, tenofovir disoproxil fumarate and lamivudine obeyed Beer's law in the concentration range of 10-60, 5-30 and 5-30 μg/ml, respectively. Results of analysis for all the three methods were analyzed and validated for various parameters according to ICH guidelines.
منابع مشابه
Development and Validation of a Rp-hplc Method for Simultaneous Estimation of Lamivudine, Tenofovir Disoproxil Fumarate and Efavirenz in a Combined Tablet Dosage Form
Objective: Development of an accurate, simple, precise and rapid method for estimating Tenofovir disoproxil fumarate, Lamivudine and Efavirenz simultaneously, in a combined tablet form. Methods: The method uses Reverse Phase High Performance Liquid Chromatography (RP-HPLC). Symmetry C18 (4.6 x 100 mm, 3.5 μm) column operated with a mixture of phosphate buffer of pH 4.0 with ortho phosphoric aci...
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